cd8 polyclonal antibody  (Bioss)

Journal: International Journal of Nanomedicine

Article Title: Ginger-Derived Exosome-Like Nanoparticles Loaded With Indocyanine Green Enhances Phototherapy Efficacy for Breast Cancer

doi: 10.2147/IJN.S478435

Figure Lengend Snippet: GDNPs@ICG suppressed breast tumor malignant behaviors. ( A ) Representative images of immunofluorescence staining of CD31 (red) and N-Cadherin (green) in mouse tumor tissue sections. The scale bar is 100 μm. ( B ) Representative images of immunofluorescence staining of IFN-γ (red) and CD8 (green) in mouse tumor tissue sections. The scale bar is 100 μm. ( C ) The expression level of IL-6 in mouse tumor tissues detected by ELISA. ( D ) Representative images of senescence-associated SA-β-gal staining (blue) in 4T1 cells after different treatments. The scale bar is 200 μm. ( E-G ) Expression of senescence-associated genes p16, p21, and p53 in mouse tumor tissues detected by RT-qPCR. n = 3, statistical significance was calculated using one-way ANOVA, and multiple comparisons were performed using the Tukey post-hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The antibodies used were N-cadherin polyclonal antibody (1:500, Proteintech, 22018-1-AP), CD31 polyclonal antibody (1:1000, Proteintech, 28083-1-AP), IFN gamma rabbit pAb (1:500, Bioss, bs-0480R), and CD8 Rabbit pAb (1:500, Bioss, bs-0648R).

Techniques: Immunofluorescence, Staining, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR